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KMID : 1094720160210030373
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Biotechnology and Bioprocess Engineering
2016 Volume.21 No. 3 p.373 ~ p.380
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Assembly of the capsid protein of red-spotted grouper nervous necrosis virus during purification, and role of calcium ions in chromatography
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Kim Hyoung-Jin
Kwag Hye-Lim
Kim Do-Gyun
Kang Bo-Kyu
Han Sang-Yoon
Moon Hyoung-Joon
Hwang Jee-Youn
Kwon Mun-Gyeong
Kang Hyun-Ah
Kim Hong-Jin
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Abstract
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Currently virus-like particles (VLPs) are receiving much attention as platforms for next generation vaccines. However, chromatography-based methods for purifying VLPs remain challenging. Unlike traditional methods using density gradient for purifying VLPs, there have been few advances in explaining how assembled particles can be obtained by chromatography. Nervous necrosis virus (NNV) infects over 30 species of fish and leads to large economic losses in the farmed fish industry. Previously we developed a heparin chromatography-based method for purifying red-spotted grouper NNV (RGNNV) VLPs. However it is unclear how the assembled RGNNV VLPs are obtained by this method. It is known that assembly of NNV capsid proteins depends on calcium ions. In the present study, we found that the yield of purified RGNNV capsid protein in heparin chromatography was enhanced when calcium ions were present during binding. Also, it appears that the capsid protein of RGNNV undergoes partial disassembly and reassembly during sample preparation prior to heparin chromatography and the protein finally undergoes assembly during the chromatography. Therefore, our results indicated that heparin-binding affinity of RGNNV capsid protein is linked to its ability for VLP formation. The assembly of RGNNV capsid proteins recombinantly produced is a good model for explaining VLP formation during chromatography-based purification processes.
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KEYWORD
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virus-like particles, assembly, nervous necrosis virus, heparin chromatography, calcium ion
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